genesee scientific/Cobalt Resin Gravity Columns/5 x 5ml/Unit/20-510G5

Prometheus™ Co-IDA Agarose resin is an affinity chromatography resin for purification of histidine-tagged proteins by Immobilized Metal Affinity Chromatography (IMAC). It binds to six histidine residues (6x His), a common tag used in protein purification and consists of iminodiacetate (IDA - a tridentate chelating agent) coupled to 6% cross-linked agarose beads. The IDA binds divalent cobalt ion with a capacity of 20-40umoles Co2+/ml resin.The protein binding capacity is >50mg protein per ml resin, but we have demonstrated binding of >130mg of purified DHAK-6XHis tagged protein to a ml of resin.

The purity and yield of the target protein varies and is dependent on many factors including working conditions, size, conformation, expression levels, solubility and sample impurities.